IGF-1 LR3 AND PEG-MGF

[Leonard Pastrana]

Can Dr. Seeds discuss the intricacies of using peptides like IGF-1 LR3 and PEG-MGF for simultaneous fat tissue loss and muscle gain.

How to cycle these peptides throughout the week in relation to different exercise modalities(resistance training vs cardio)

How to time the administration correctly in regards to nutrition intake and exercise.

What to look out for in regards to the length of treatment and dosing.

 

[Kristelle Reyes]

Hello doctors @byurthgmail-com @drerikaeshealth-com @drhusaininterlinkedmd-com @drkriswyahoo-com @cpaigepaigemd-com , would love to have your inputs here. Thank you! ?

[Madison Lepore]

Hello Leonard!

Think of MGF (IGF-1-EC) as a proliferation peptide and IGF-1 LR as a differentiation peptide when focusing on the satellite cells of muscle. These satellite cells are stem cells of muscle repair and they are necessary for muscle hypertrophy from resistance exercise. These stem cells fuse with the traumatized muscle cells from resistance exercise. With the guidance of MGF (IGF-1-EC) and IGF-1 LR they develop into essentially new muscle cells (these are not new cells they are muscle cells that have fused with the satellite cell to create a new hypertrophy cell, but they are not brand new muscle cells. We are not grossly increasing the number of new muscle cells we are re-engineering the muscle cells we have present.)

MGF (IGF-1-EC) is a peptide that sits in muscle and activated by breakdown of muscle. Will only be activated if you breakdown muscle after resistant type of exercise. Aerobic exercise typically doesn’t break down but HIIT (depends on the HIIT exercise) may cause some breakdown. MGF (IGF-1-EC) activates stem cells (satellite cells) to repair and they help muscle rebuild. Proliferation phase begins with muscle breakdown or muscle stretch and this involves the release of  MGF (IGF-1-EC) . IGF-1 LR initiates maturation and differentiation. IGF-1 LR takes over later in recovery (1 day later).

MGF (IGF-1-EC) increases the expression cell cycle progression factors. IGF-1 LR promotes myoblast differentiation as they come out of the cell cycle. Remember the stem cell has fused to the injured muscle cell, which we are referring to as the myoblast.

Isoform IGF-1-EC (mechanical growth factor) increases in exercised muscle as compared to resting state. MGF (IGF-1-EC) has been known to stimulate satellite cells to enter the cell cycle and proliferate.

DAY 1-Training day: MGF (IGF-1-EC) BEFORE exercise. You can dose up to 100mcg/day. The dosing can be split between muscle groups. IE chest day inject 50mcg per pec or 25mcg per pec and 25mcg per bicep to total 100mcg.

DAY 2-Recovery day: Inject IGF-1 LR in muscle groups working on (ie chest day inject in chest). Dosing is up to 100mcg that day. Can split the dosing the same way as IGF-1 LR. Can go as low as 25mcg. You can spilt the dosing however you want. Next day do the same dose. Recovery day needs to be a non-resistance day. It can be aerobic.

IGF-1 LR3 and MGF (IGF-1-EC) are not affected by nutrition. In fact they can be taken with a carbohydrate or protein to avoid hypoglycemia.

A muscle building Cycle is 6-8 weeks. You can potentially extend to 3 months for significant bulking but 8-12 weeks in the MAXIMUM cycle to be considered to avoid organomegaly.

A typical cycle utilized is between 6-8 weeks with 6 weeks off and then repeated as necessary.

Here is a reference paper for more information that shows relevant pathways and the basic science of  IGF-1 that you should consider reading. IGF-1-Mediated Regulation of Skeletal Muscle Hypertrophy and Atrophy: Yoshida, Tadashi, and Patrice Delafontaine. “Mechanisms of IGF-1-Mediated Regulation of Skeletal Muscle Hypertrophy and Atrophy.” Cells 9, no. 9 (August 26, 2020): 1970. https://doi.org/10.3390/cells9091970.

[William Seeds, MD]

@leonard-pastranagmail-com tagging you!

and thank you @madisonseeds-md! =)

[Leonard Pastrana]

@madisonseeds-md Thank you so much! Very much appreciate the link to this reference paper as well. Looking forward to this read.

[Marc Rodriguez]

@madisonseed-md Wow, what a great explanation and verbal illustration. Are you Dr.Seeds daughter?

[Kristelle Reyes]

Claim your CME here: https://earnc.me/mU9g1c

[beth panoff]

In going back over this I have a question regarding utilizing the PEG MGF and IGF1 LR- in working with post menopausal females to optimize lean mass and hopefully build some more muscle too of course all hormones are already optimized etc.

Is there any benefit to utilizing just one of these and if so how do you optimize the dosing/timing?

Obviously utilizing both would be best. How wold you add in cic/ipa if at all.

Thank you!

Beth

[PATRICK FREE]

Wow, that was an excellent and very informative answer @madisonseeds-md. I have a patient that sustained torn quadriceps while working out and I am putting together a regiment to aid in the healing and recovery process. I will be including TB4, BPC, CJC/ipamorlin, as well as some oral supplementation.  I was curious about adding in the PEG-MGF, to increase the satellite stem cell pool and aid in the healing process. What are your thoughts on adding this to the regimen, in this specific scenario? Thank you.

[William Seeds]

Excellent question @free703msn-com!

patrick let me know if sets you in the right direction . I think this can be a useful peptide .

 

MFG is a peptide that mimics an Isoform if IGF-1EC made by the liver that is very active in satellite cell activation .

Here is a brief section of a book I am writing on specific to what I am referring to ;

In summary, IGF-1EC is a splice variant of IGF-1 that is produced in response to mechanical stress, such as that induced by exercise or injury. It promotes muscle protein synthesis, activates intracellular signaling pathways involved in muscle growth and differentiation, and stimulates the proliferation and differentiation of satellite cells. These mechanisms make IGF-1EC an important player in muscle rebuilding after resistance exercise.

Research also shows the role of MGF in satellite cell activation and muscle repair and recovery. MGF (mechano growth factor) is another  splice variant of insulin-like growth factor-1 (IGF-1) that is produced locally in muscle tissue in response to mechanical stress, such as that induced by resistance exercise or muscle injury (Goldspink, 2005). MGF is a potent activator of satellite cells, which are muscle stem cells that play a critical role in muscle growth, repair, and regeneration (Hill et al., 2003).

Studies have suggested that MGF may play an important role in muscle repair and recovery by stimulating satellite cell activity. For example, a study by Hameed et al. (2008) found that MGF expression increased in response to resistance exercise, and that this increase was associated with increased satellite cell activity. Another study by Kadi et al. (2005) found a correlation between MGF expression and muscle fiber growth in response to resistance exercise.

MGF has also been investigated for its potential use in treating muscle injury and wasting conditions. For instance, a study by Hill and colleagues (2003) found that MGF injection improved muscle regeneration and function in a model of muscle injury in rats. More recently, a study by Wang and colleagues (2017) reported that MGF treatment improved muscle regeneration and recovery in a mouse model of muscle atrophy and impaired regeneration.

In summary, MGF is a potent activator of satellite cells, which play a critical role in muscle growth, repair, and regeneration. Studies suggest that MGF may play an important role in muscle repair and recovery following exercise or injury, and that it may have potential therapeutic applications for treating muscle injury and wasting conditions.

MGF appears to play a role in the early stages of muscle repair and regeneration by promoting the proliferation and differentiation of satellite cells, which are a type of muscle stem cell that are important for repairing and building new muscle tissue. Research suggests that MGF levels increase rapidly in response to mechanical stress, such as that caused by resistance exercise or muscle injury, and that MGF can stimulate satellite cell activation and muscle growth (Hill et al., 2003; Goldspink, 2005).

IGF-1, including its specific spliced variant IGF-1EC plays a role in promoting muscle growth and repair, but it may have a more important role in later stages of muscle cell maturation and hypertrophy. IGF-1 is thought to act by promoting muscle protein synthesis and activation of intracellular signaling pathways involved in muscle growth, and IGF-1EC may promote satellite cell activation similar to MGF (Blanquiceth et al., 2021; Chen et al., 2020).

 

References:

Blanquiceth, Y. R., Gomes, I. A., & Pimentel, G. D. (2021). Insulin-like growth factor-1 splice variants in the muscle: Implications for regeneration, hypertrophy, and plasticity. International Journal of Molecular Sciences, 22(9), 4509. https://doi.org/10.3390/ijms22094509

Chen, J. L. W., Walton, K. L., Hagg, A., & Colgan, T. D. (2020). Defining the roles of IGF-I splice variants in muscle-building & pro-survival: A review. Journal of Cachexia, Sarcopenia and Muscle, 11(4), 870–886. doi: 10.1002/jcsm.12541

Goldspink, G. (2005). Mechanical signals, IGF-I gene splicing, and muscle adaptation. Physiology, 20(4), 232-238. https://doi.org/10.1152/physiol.00005.2005

Hill, M., Wernig, A., Goldspink, G., & Muscle Study Group. (2003). Muscle satellite (stem) cell activation during local tissue injury and repair. Journal of Anatomy, 203(1), 89-99. https://doi.org/10.1046/j.1469-7580.2003.00219.x

Wang, X. Q., Edens, N. K., Heiny, J. A., Zhao, J. Y., & Ding, Y. (2017). Rodent models for studying muscle regeneration. Current Protocols in Mouse Biology, 7(4), 259-278. https://doi.org/10.1002/cpmo.28

Hameed, M., Toft, A. D., & Harridge, S. D. R. (2008). Growth hormone and IGF-I responses to resistance exercise during puberty: Implications for muscle mass and bone dimensions. The Journal of Clinical Endocrinology & Metabolism, 93(3), 723-727. https://doi.org/10.1210/jc.2007-2009

 

[beth panoff]

If an individual is trying to put on muscle and they only have one recovery day do they only then do IGF-1 once a week?

I have a 30-year-old female who is looking to put on a muscle and I’m thinking of using MGF and IGF -1 but not completely clear on how and when to give the IGF-I if she is doing five days of resistance training.
Any  guidance?

[William Seeds]

 

Beth @bpanoff1010gmail-com,  good question , so if you are using these peptides i would use them site specific the  body part they are working on . It can be extremely complicated when trying to use these peptides for people who train in this fashion. so you have options of just concentrating on particular muscle group. For instance if this person trained legs on monday you could use MGF the day of the work out muscle specific ( quads) and then tuesday use the IGF-1LR  same area of the quads  and repeat this the next week and do this for 6-8 weeks , no mater what exercise she is doing tuesday, wed, thur, friday , sat.   This is the ideal way to be muscle specific especially when using MGF.  you will have great success with site specific and tailored approach

good luck

[Anthony Castore]

This is a fantastic thread and I am grateful for the people who have contributed on here and given me the opportunity to learn.  I am a strength coach who works with doctors and I have been fascinated by all aspects of performance. I do not have the medical credentials but, a fierce passion for learning and (for better or worse) a lab rat mentality.  I work with a number of physicians in the central Ohio area and I regularly get labs run so I am not wreckless, just driven.

With that out of the way I would like to offer a little insight as to how I have used these peptides both as part of a repair program that brough me back from a torn (in the belly) pec in 4 weeks and how I incorporate them into protocols for myself and others I have worked with who have a goal of building muscle and losing fat.

 

Here is the recovery protocol I have used:

TB-500, BPC-157 and Peg-MGF Recovery Protocol.

* TB-500: 500mcg per day before bed

* BPC-157: 250mcg Twice daily AM/PM

* Peg MGF: 200mcg intramuscular site injection, M,W,F (best if used on non workout days or at least 2 hours after workout as it competes for receptors with IGF and loses)

⭐ For even better effect:

* Add HGH at 2.5-4iu per day. Split daily dose into 2 intramuscular injections, AM/PM

* IGF-DES at 50mcg twice daily, intramuscular site injection.

NOTES

1. THIS IS NOT A CURE ALL! However, it will greatly speed recovery of damaged tissue from burns and lacerations, muscle tears and connective tissue damage, tendinitis, sprains, hairline fractures, impact injuries and serious bruising and will also slow the degeneration of cartilage due to Osteoarthritis!

2. Use a small gauge insulin needle (30-31 gauge, 5/16”)

3. Reconstitute vials with 1ml of water

4. Peg MGF and IGF-DES have site specific effects

5. For site injections: Draw the appropriate amount of suspension into your needle and backfill the remainder of the space within the syringe with bacteriostatic water. This will dilute the suspension and add more volume which will allow you to saturate the target area with several small “micro injections” for best effects.

6. This protocol is typically run for 6-8 weeks or until fully recovered at which point, discontinue the Peg MGF and IGF DES. Then drop to a maintenance dose of TB-500 at 500mcg once per week and 250mcg of BPC-157 every other day. The length of the maintenance phase is user determined and can easily and safely become part of the daily routine.

 

For the training for fat loss and hypertrophy….

Let’s first clarify the types of muscle hypertrophy:

• Sarcoplasmic hypertrophy
• Myofibrillar hypertrophy
• Mechanical tension-induced hypertrophy
• Mechanotransduction hypertrophy

1. Sarcoplasmic Hypertrophy
   • Stimulus: High volume, lower intensity resistance training with short rest intervals.
   • Training effects: Increased muscle size, but not as much functional strength, as it primarily increases the fluid (sarcoplasm) within muscle cells.
   • Hypothetical arm workout routine: 4 sets of bicep curls, 12-15 repetitions with light to moderate weights, short rest periods of 30-60 seconds.
   • Macronutrient and Substrate Requirements: High carbohydrates for energy during longer-duration workouts, moderate protein to support muscle repair and growth, low to moderate fat.
2. Myofibrillar Hypertrophy
   • Stimulus: Low volume, high intensity resistance training (heavy lifting), longer rest intervals.
   • Training effects: Increases in strength and density of muscle fibers, more functional strength but less visible size increase than sarcoplasmic hypertrophy.
   • Hypothetical arm workout routine: 5 sets of 5 reps of heavy bicep curls or weighted pull-ups, with 3-5 minute rest periods.
   • Macronutrient and Substrate Requirements: High protein to support muscle growth and repair, moderate carbohydrates for replenishing glycogen stores, moderate to high fat for hormone regulation and energy.
3. Mechanical Tension-Induced Hypertrophy
   • Stimulus: Any form of resistance training that emphasizes time under tension and progressive overload.
   • Training effects: Increase in both muscle size and strength, as mechanical tension can stimulate both myofibrillar and sarcoplasmic hypertrophy.
   • Hypothetical arm workout routine: 3 sets of 8-10 repetitions of bicep curls with slow, controlled movement (4 seconds up, 4 seconds down), 1-2 minute rest periods.
   • Macronutrient and Substrate Requirements: High protein for muscle repair and growth, moderate carbohydrates for energy and glycogen replenishment, moderate fat for energy and hormone regulation.
4. Mechanotransduction Hypertrophy
   • Stimulus: External forces (mechanical loads) being sensed and converted into biochemical signals that result in muscular adaptation.
   • Training effects: Increases muscle size and strength, as mechanotransduction can stimulate both myofibrillar and sarcoplasmic hypertrophy.
   • Hypothetical arm workout routine: Any progressive overload-focused training can stimulate mechanotransduction, for example, adding more weight to bicep curls or pull-ups each week, 3 sets of 8-10 repetitions, 2-3 minute rest periods.
   • Macronutrient and Substrate Requirements: Balanced intake of high protein for muscle repair and growth, moderate carbohydrates for energy and glycogen replenishment, moderate fat for energy and hormone regulation.

In summary, each type of hypertrophy has a different stimulus and leads to different effects on muscle size and strength. The workout routines can be similar but the specific parameters (like the weight, repetitions, and rest periods) can vary. Nutrition needs also vary based on the type and intensity of the training.

 

With these as the different training options for fat loss I generally like to do a sarcoplasmic hypertrophy routine with a 10% caloric deficit and 50% of the carbohydrates peri-workout and the remaining carbohydrates later in the day. In this case, I am trying to optimize uptake of glucose in cells while minimizing the demand on the CNS.

One Peptide protocol I have used in this case would be:

-30mg MOTSc/day on training days

-1mg tesamorlein morning empty stomach/ before bed empty stomach

-1x every 15 days ACE 031 3mg/kg every

-Pre workout IGF DES 100mcg split into trained muscles

-500mg BPC157 Frag TB4 500mg bid (post workout meal and before bed)

on non training days (at the same time I would normally be working out) peg MGF 200mcg split into trained muscle groups.

As part of our holistic training regimen, we integrate tailored nutrition supplementation to complement each training session. This typically includes plasmalogens, fatty 15, and Gluco IR from NutraDyn, accompanied by an intra-workout mineral/electrolyte high molecular weight (HMW) carbohydrate amino supplement. After workouts, our focus shifts towards re-establishing immune responses where we utilize supplements such as Manuka honey and lactoferrin, in addition to the protein-carb supplement that best addresses individual needs.

I’m excited to share that this year we’re preparing to launch a series of courses. These courses are intended to build a bridge between strength coaches and the medical community, highlighting the crucial interaction between training, nutrition, and supplementation. I believe in the importance of a comprehensive, team-based approach to health and fitness, which includes working closely with qualified medical practitioners.

Consider this: a typical person visits their doctor 2-4 times a year, yet that same individual interacts with me, their trainer, 2-4 times a week. Imagine the transformative potential if we could enhance trainers’ knowledge and connect them with forward-thinking medical professionals. Together, we could amplify our impact on health and training outcomes. Trainers could then serve as vigilant observers, logging valuable data based on their day-to-day interactions and progress notes, making healthcare delivery more efficient.

I am passionate about this vision, and I am open to hearing any thoughts, ideas, or even criticisms you may have about this approach. We are all here to learn and to grow, and your expert feedback is essential for that process. If anyone is interested in exploring this further with me, or simply wishes to provide feedback, I wholeheartedly welcome it.

Thank you for allowing me to be a part of this exceptional community, and for the wealth of knowledge that SSRP consistently provides through its faculty, teachings, and members. I am thrilled to be on this journey with you all, learning and shaping the future of health and fitness together.

[Rita Ferraro, ND]

Anthony, Will you be in Malibu at the World Congress in August?

[Anthony Castore]

I am hoping to go. I haven’t been able to sign up with the link on the member site so I reached out for support. If there is still availability and I am able to register I wouldn’t miss it!! I am really looking forward to the chance to learn and network. Will you be attending?

[Author]

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