Humanin & Pinealon

[Robb Bird]

Anyone worked with these, any dose protocols out there?

thanks

[Anthony Castore]

Pinealon

Pinealon, a peptide known for its potential neuroprotective benefits, is sparking interest in scientific circles due to its unusual ability to interact directly with DNA. This sets it apart from most other peptides, which generally work through surface or cytoplasmic receptors. Here’s a deep dive into how pinealon influences cell function at the genomic level and why this matters.

Unlike typical peptides that stay outside the cell nucleus, pinealon is small enough to cross both cellular and nuclear membranes, giving it direct access to DNA within cell nuclei. Studies on HeLa cells have demonstrated pinealon’s capacity to penetrate these membranes, allowing it to interact directly with genetic material. This unique property is likely due to pinealon’s very small molecular size, enabling it to slip through lipid bilayers with ease.

Once inside the nucleus, pinealon appears to regulate gene expression. This is significant because most peptides influence cells indirectly through receptors; pinealon’s direct approach allows it to affect cellular processes more broadly and potentially with greater specificity. This ability to influence gene expression could explain pinealon’s diverse biological effects, some of which aren’t explained by traditional receptor-based mechanisms.

Pinealon also exhibits dose-dependent effects, meaning its impact varies with concentration. Studies show that:

At lower doses, pinealon helps limit the build-up of reactive oxygen species (ROS), which are harmful byproducts of cellular metabolism, and reduces cell death rates.

At higher doses, it continues to influence the cell cycle even after its antioxidant benefits have been fully utilized.

This dose-dependence hints at pinealon’s direct genomic interaction, as it seems to play a broader role than just an antioxidant.

Pinealon might promote the expression of 5-Tryptophan Hydroxylase which is necessary for serotonin synthesis, potentially enhancing mood and cognitive function through epigenetic modifications.

Pinealon may also affect irisin production in muscle cells. Irisin is associated with fat metabolism and telomere length, linking pinealon to cellular processes involved in aging and metabolic health.

Pinealon’s role extends to cell cycle regulation, where it appears to activate pathways that support cell proliferation. Under oxidative stress, however, this doesn’t necessarily lead to more cell division; instead, it seems to offset some of the damage caused by ROS, helping cells maintain their vitality and function under challenging conditions.

Pinealon’s ability to directly engage with DNA and modulate gene expression is a rare trait among peptides. This direct genomic influence enables pinealon to impact a range of cellular processes, from antioxidant protection to cell cycle regulation, in a way that depends on its concentration. While these findings are promising, more research is essential to clarify the exact genes and pathways affected by pinealon and to fully understand its potential benefits and risks.

Pinealon is typically administered via subcutaneous injection or intraperitoneal injection.

The effective dose range is generally 10-200 μg/kg body weight. Some common doses used in studies include:

– 10 μg/kg
– 50 μg/kg
– 100 μg/kg
– 200 μg/kg

– Daily administration for 5 consecutive days is a common protocol
– Some studies use every other day administration for 5-10 total doses

– Short-term use of 5-10 days is most common
– Some studies have used longer durations up to 1 month

Morning administration is most commonly reported, though the optimal time of day has not been definitively established.

Based on the available evidence, an effective protocol appears to be:

– 50-100 μg/kg dose
– Administered subcutaneously
– Once daily in the morning
– For 5 consecutive days

This dosing regimen has shown beneficial effects in multiple studies while minimizing potential side effects. However, the optimal protocol may vary depending on the specific condition being treated. I have personally had good success for improving REM sleep taking 10mg/day for 10 days straight.

Humanin

Humanin, a peptide derived from mitochondria, is generating interest for its impressive anti-inflammatory effects, particularly in the brain. Emerging research highlights its potential to combat inflammation on multiple fronts, from reducing pro-inflammatory markers to supporting cellular defenses against oxidative stress. Here’s a closer look at how Humanin could benefit brain health and help manage neuroinflammation.

One of Humanin’s standout effects is its ability to lower levels of pro-inflammatory markers, particularly in Alzheimer’s disease models. Research has shown that Humanin treatment decreases pro-inflammatory cytokines like TNF-α and IL-6, which are heavily implicated in neuroinflammation. Humanin also helps dampen inflammation triggered by amyloid-beta, a protein associated with Alzheimer’s, and reduces the activation of reactive astrocytes and microglial cells, both key players in the brain’s inflammatory response.

Microglia are the brain’s resident immune cells, and they play a crucial role in managing inflammation and cleaning up cellular debris. Humanin appears to encourage a “reparative” state in microglia, boosting their phagocytosis (the process of engulfing harmful particles) and reducing pro-inflammatory signals. This means Humanin doesn’t just suppress inflammation but actively supports the brain’s repair processes, even aiding in clearing hematomas (bleeds) after brain injuries.

Oxidative stress, which damages cells and accelerates aging, can fuel inflammation. Humanin provides a protective buffer by helping shield cells, including endothelial cells, from oxidative stress. By preventing the initial cellular damage, Humanin indirectly limits the downstream inflammatory response—a proactive approach to keeping inflammation in check.

One fascinating aspect of Humanin is its interaction with specific receptors involved in inflammation. It competes with amyloid-beta to bind to the G protein-coupled receptor FPRL1 (or FPR2 in mice), which amyloid-beta often uses to initiate inflammatory responses. By blocking amyloid-beta’s access to these receptors, Humanin helps prevent the chain reaction of inflammatory activation.

In aging studies, Humanin’s benefits aren’t limited to the brain. Research in older mice reveals that Humanin can also reduce inflammation markers circulating throughout the body. This suggests that Humanin’s anti-inflammatory reach could extend beyond the brain, potentially impacting overall health and longevity.

Humanin doesn’t just fight inflammation directly; it also shields neurons from damage and death. By protecting neurons, Humanin may help reduce the secondary inflammatory response that typically follows neuronal injury. This neuroprotective effect could be particularly valuable in managing conditions where cell death and inflammation go hand in hand.

As a mitochondrial-derived peptide, Humanin naturally supports mitochondrial function. Healthy mitochondria produce less oxidative stress, which in turn means less inflammation. By enhancing mitochondrial performance, Humanin offers a foundational approach to maintaining cellular health and reducing chronic inflammation.

Humanin stands out as a mitochondrial peptide with the unique ability to tackle inflammation from multiple angles. Whether by directly interacting with inflammatory cells, blocking inflammatory pathways, or supporting the health of mitochondria and neurons, Humanin offers a promising tool for addressing neuroinflammation.

Here is a summary of the most effective dosing protocol for Humanin:

The effective dose range appears to be 1-4 mg/kg body weight. Some common doses used in studies include:

– 1 μg/g (1 mg/kg)
– 2 mg/kg[4]
– 252 μg/kg (0.252 mg/kg)

Humanin and its analogues are typically administered via:

– Intravenous injection
– Intraperitoneal injection
– Intranasal administration

Subcutaneous injection may also be effective, though it was not specifically mentioned in the search results.

– Daily administration for 7 consecutive days is a common protocol
– Every other day administration has also been reported
– Some studies used two doses per day – one during ischemia and one at reperfusion.

For longer-term use, I like 5 mg every 4-5 days or 2 mg every other day.

Morning administration is most commonly reported, though the optimal time of day has not been definitively established. For treating ischemia/reperfusion injury, administration 15 minutes after ischemic onset and at the onset of reperfusion was effective.

Best Overall Protocol:
Based on the available evidence, an effective protocol appears to be:

– Dose: 1-2 mg/kg
– Route: Intravenous or intraperitoneal injection
– Frequency: Daily or every other day
– Duration: 7-14 days
– Timing: Morning administration

I personally use this as part of a rotation of mitochondrial peptides at a subQ dose of 2mg for 10days.

 

[Robb Bird]

Thank you Anthony for your time and insight, much appreciated!

[Anthony Castore]

Happy to help! There are so many brilliant minds in here. It is a great community.

[Eric Fete]

For sure!

Anthony I would love to connect sometime;lots of shared interests

 

Eric

740-704-5386

 

[Anthony Castore]

Eric,

It would be great to connect! I believe we ware both in Ohio! I will send you a text with my contact and let’s figure out a good time to catch up! I am looking forward to it

[Kristelle Reyes]

Hello @drbirdtransformyou-com,

Here’s a video response from Dr. Seeds’ December Office Hours at 13:08. Please watch by clicking the link below:

https://ssrpinstitute.org/ssrp-members-videos/

Thank you! ?

[Author]

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